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  • "Pickles" started this thread

Posts: 769

Reg: Dec 30th 2007

Location: South London

Children: Was Lister

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1

Saturday, February 14th 2009, 6:18pm

NK cells test - help with results

A while a go I had tests for NK cells and while I was told that I had high killer cells I wasn't sure of the detail.

I've just had my notes photocopied and can now see the detail behind my results but I don't understand them and I can't find the info on any other threads - can anyone help.

My total NK cells count is 147.2
Total NK %9.7%
Total NK CD69 absolute counts 2.26
Total NK CD69 % 1.53

NK cells cytotoxix assay%
NK cell 50:1 25

From what I remember the last figure of 25% is the one that comes down on steroids to 14% but I've no idea what the others means and why I had CD69 cells while others on this thread have different numbers.

Thanks for any advice given
Michele




4 ICSI - all BFN
Poor responder (6 eggs max on left ovary only)
High NK cells
Considering Egg Donation

Fancellu

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Posts: 41

Reg: Oct 10th 2008

Location: Epsom

Children: 3 years old son

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2

Saturday, February 14th 2009, 6:30pm

RE: NK cells test - help with results

Michele,

Your NK results is different from the Chicago lab and the Alan Beer Lab. I did the NK test on NHS by Dr Shehata, the test only measures CD69,my result was normal. I did the NK test again at Alan Beer Lab a couple of months later, and my CD56 is high and CD19 is borderline.

Where did you do the test? I think the NK test here is different from US.

Jenny

  • "Pickles" started this thread

Posts: 769

Reg: Dec 30th 2007

Location: South London

Children: Was Lister

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3

Saturday, February 14th 2009, 6:39pm

I had mine done at the Lister and thought this would be good enough - it cost us about £500!

At the time I was none the wiser and didn't know there were different methods of testing. I've had three ICSI with no implanation so thought NKs might be an issue.




4 ICSI - all BFN
Poor responder (6 eggs max on left ovary only)
High NK cells
Considering Egg Donation

Fancellu

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Posts: 41

Reg: Oct 10th 2008

Location: Epsom

Children: 3 years old son

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4

Sunday, February 15th 2009, 11:37am

Michele,

Have done these tests:

1. DQ Alpha (for you and your partner)
2. LAD
3. Th1/Th2 ratio
4. inherited blood clotting disorder

Jenny

kar1

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5

Sunday, February 15th 2009, 12:26pm

it should say on your result what the upper or lower levels are

test results tend to have <?? on them
TTC 12 years

2 early losses

lots of ops and tx

both tubes removed

5th fresh short protocol
:BFP:

Tyler May born 5/5/2010 by emergency c section. Tyler is our sunshine

  • "Pickles" started this thread

Posts: 769

Reg: Dec 30th 2007

Location: South London

Children: Was Lister

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6

Sunday, February 15th 2009, 12:33pm

Do you mean this:

Total NK 147.2 (below 200)
Total NK% 9.7 (1-12)
Total NK CD69 absolute counts 2.26 (below 1.0)

Does all of that info lead to the cytotoxic assay of 25%?




4 ICSI - all BFN
Poor responder (6 eggs max on left ovary only)
High NK cells
Considering Egg Donation

kar1

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7

Sunday, February 15th 2009, 12:42pm

i don't know hun

its hard to say because it depends on the where the results came from (which lab)

do you know if you have it testing with steriods and ivig?
TTC 12 years

2 early losses

lots of ops and tx

both tubes removed

5th fresh short protocol
:BFP:

Tyler May born 5/5/2010 by emergency c section. Tyler is our sunshine

Posts: 2,643

Reg: Feb 28th 2007

Location: Surrey

Children: Not yet

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8

Sunday, February 15th 2009, 1:50pm

Hi There

I don't understand your readings, I had a similar test done here in the UK a couple of years ago and was told (like Fancellau) that mine were normal. When I had a full assay done with Alan Beer in US I was found to have high NK's which were tested against IVIG only.

Annoyingly the ones done here in the UK are not very readable and can only be read by a specialist, plus they only look at one marker so not very detailed.

The important % or number is the overall killing power of them, so in the US ones this would be the top figure at 50:1, this ideally needs to be below 10%.

x
TTC naturally, five miscarriages 2006-2009, Immune issues

BLONDIE'S IMMUNOLOGY DIARY

  • "Pickles" started this thread

Posts: 769

Reg: Dec 30th 2007

Location: South London

Children: Was Lister

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9

Sunday, February 15th 2009, 3:41pm

I was told I had high NK cells which is why I was on steroids last time but I had wanted to try and make sense of the readings. I've not had IVIG.

I just wanted to see if I could learn a bit more about the test but I guess it varies too much between tests.

Thanks anyway




4 ICSI - all BFN
Poor responder (6 eggs max on left ovary only)
High NK cells
Considering Egg Donation

kar1

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  • "kar1" is no longer a member of FZ

Posts: 12,830

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10

Sunday, February 15th 2009, 4:22pm

what steriod and what dose have you used?

you could take more or ask for intrilipids
TTC 12 years

2 early losses

lots of ops and tx

both tubes removed

5th fresh short protocol
:BFP:

Tyler May born 5/5/2010 by emergency c section. Tyler is our sunshine

  • "Pickles" started this thread

Posts: 769

Reg: Dec 30th 2007

Location: South London

Children: Was Lister

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11

Sunday, February 15th 2009, 10:00pm

I was on 20mg of pendisolene (sorry that's from memory and not looking on the box).

I've not heard of intripilides?




4 ICSI - all BFN
Poor responder (6 eggs max on left ovary only)
High NK cells
Considering Egg Donation

Posts: 2,643

Reg: Feb 28th 2007

Location: Surrey

Children: Not yet

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12

Monday, February 16th 2009, 11:50am

Pickles just so you know that steroids do not always work on NK cells, it really does depend on your levels.

I too had 25mg prednisalone and still miscarried but mine are high at 21% killing power. This is when you would need further treatment such as IVIG or Intralipids.

x
TTC naturally, five miscarriages 2006-2009, Immune issues

BLONDIE'S IMMUNOLOGY DIARY

JENSQUI

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Posts: 7,427

Reg: Feb 26th 2007

Location: Edinburgh

Children: one very special daughter

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13

Monday, February 16th 2009, 1:47pm

Hi Pickles,

I don't know anything about this stuff myself, but there is a thread on intralipids which may help:
Information on Intralipids

xx


me 38, DP 44, ttc since 2005
BFP May 07 - HB seen @ 8 wks but CRL only 7 weeks, m/c @ 9 wks
BFP Jan 08 -m/c @ 11 wks (D&C)
BFP Jun 08 - early loss
BFP Dec 08 - HB & CRL ok @ 8 wks, but sac too small, m/c @ 9 wks
BFP Apr 09 - Martha born 9th Jan 2010
BFP Feb 12 - Eliza born 13th Oct 2012




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